EC Number |
Storage Stability |
---|
1.2.1.10 | -60°C, 20 mM MOPS-KOH pH 7.6, 0.1 mM EDTA, up to one year |
1.2.1.10 | -70°C, 10 mM Tris-HCl buffer, pH 8, 3 mM dithioerythritol, 2 weeks, 15% loss of activity |
1.2.1.10 | -70°C, 3 mM dithioerythritol, 85% of enzymatic activity remains after 2 weeks |
1.2.1.10 | -80°C, 10 mM phosphate buffer, pH 7.5, 1 mM dithiothreitol |
1.2.1.10 | 3°C, ammonium sulfate step of purification, several months |
1.2.1.10 | 4°C, anaerobically |
1.2.1.10 | 5 mM dithiothreitol protects enzyme in crude extracts from O2-inactivation for at least 2 hours |
1.2.1.10 | addition of 140 mM KCl to 10 mM Tris acetate stabilizes as no activity is lost after 3 days |
1.2.1.10 | CoA and dithiothreitol restore a higher activity than one of these compounds alone |
1.2.1.10 | glycerol at 20%, vol/vol, stabilizes in 50 mM Tris acetate buffer at pH 7, as no activity is lost after 3 days at 4°C under argon |