EC Number |
Storage Stability |
---|
1.13.12.6 | -15°C, distilled water |
1.13.12.6 | -80°C, lyophilized Cypridina luciferin is stable for more than 1 year and the enzyme in acidic ethanol solution for at least 1 month |
1.13.12.6 | 4°C, PBS buffer containing 0.1 M potassium phosphate (pH 7.2) and 0.15 M NaCl, 2 months, without significant loss of activity |
1.13.12.6 | Cypridina luciferase occasionally loses almost all of its activity when chromatographed through the 90-cm Sephadex column, irrespective of whether the column is newly poured or has been used for a period. The loss also occurs with both newly dissolved and old solutions of Cypridina luciferase |
1.13.12.6 | purified native Apogon luciferase gradually loses activity on standing in the cold over a period of weeks |