EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    6.3.1.5 | 0.0003 |
- |
mutant enzyme R112L, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.0011 |
- |
mutant enzyme D593A/F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.0027 |
- |
mutant enzyme L604A/L519A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.0033 |
- |
mutant enzyme Y532A/M621A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.0043 |
- |
mutant enzyme L604N, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.008 |
- |
mutant enzyme F622A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.009 |
- |
partially purified recombinant His-tagged enzyme expressed in insect cells, substrate NH4+ |
653750 |
| 6.3.1.5 | 0.0143 |
- |
mutant enzyme I111A, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.015 |
- |
mutant enzyme R112S, at 37°C, with 2 mM ATP, 5 mM MgCl2, 50 mM Tris-HCl, pH 8.0, 56 mM KCl, and 0.2 mg/ml bovine serum albumin |
674687 |
| 6.3.1.5 | 0.033 |
- |
purified recombinant HIs-tagged enzyme expressed in Escherichia coli, substrate glutamine |
653750 |
