EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   4.2.1.30 | -999 |
- |
- |
33818, 33819, 33820, 33823, 33825, 33826, 33828, 33830, 33837 |
| 4.2.1.30 | -999 |
- |
a reliable, enzyme-coupled assay for measuring glycerol dehydratase activity in crude-cell extract is developed using 1,2-propanediol as the substrate. In the assay, 1,2-propanediol is converted to propionaldehyde, which is quickly converted to 1-propionic acid by aldehyde dehydrogenase (with the production of NADH) or to 1-propanol by yeast alcohol dehydrogenase (with the consumption of NADH). The change in NADH concentration, as monitored at 340 nm spectrophotometrically, manifested as a straight line for 3 min, from which the glycerol dehydratase activity can be determined. The assay method should prove to be applicable to recombinant strains developed for the production of 3-hydroxypropionic acid and/or 1,3-propanediol from glycerol |
748353 |
| 4.2.1.30 | -999 |
- |
spectrophotometric assay for the sensitive measurement of the glycerol dehydratase activity with a sub-nanomolar limit of detection. The assay method employs aldehyde dehydrogenase as a reporter enzyme, so the readout of the glycerol dehydratase activity is recorded at 340 nm as an increase in UV absorbance which results from NADH generation accompanied by oxidation of 3-hydroxypropionaldehyde to 3-hydroxypropionic acid. The glycerol dehydratase assay is performed under the reaction conditions where the aldehyde dehydrogenase activity overwhelms the GDHt activity (i.e., 50fold higher activity of aldehyde dehydrogenase relative to glycerol dehydratase activity), affording sensitive detection of glycerol dehydratase with 360 pM limit of detection |
748257 |
| 4.2.1.30 | 0.2 |
- |
- |
672728 |
| 4.2.1.30 | 0.299 |
- |
recombined glycerol dehydratase |
705190 |
| 4.2.1.30 | 1.3 |
- |
- |
33834 |
| 4.2.1.30 | 4 |
- |
- |
33833 |
| 4.2.1.30 | 21.2 |
- |
crude extract, at pH 8.5 and 45°C |
725951 |
| 4.2.1.30 | 65.4 |
120 |
dependent on purification method |
651133 |
| 4.2.1.30 | 91.9 |
- |
- |
673100 |
