EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
4.2.1.104 | -999 |
- |
- |
210672 |
4.2.1.104 | -999 |
- |
activity assay in 0.1 M potassium phosphate buffer at pH 7.5 and 37°C with or without 3 mM NaHCO3, cyanate concentration between 0.2 mM and 2 mM, 0.2 to 1 microgram total protein per milliliter, measurement of NH3 formation |
678457 |
4.2.1.104 | -999 |
- |
cyanase activity shown in whole-cell extracts of Synechocystis, high level of cyanase activity when grown with nitrate as N source but unable to utilize exogenous cyanate to support O2 evolution in the light |
680479 |
4.2.1.104 | -999 |
- |
cyanase activity shown in whole-cell extracts of the photoautotrophic Synechococcus sp. strain UTEX 625, ability to utilize cyanate-derived CO2 to support photosynthesis affected |
680479 |
4.2.1.104 | -999 |
- |
strain 21 of Pseudomonas putida is a more active CN– decomposer than strain 18 of Pseudomonas stutzeri |
681808 |
4.2.1.104 | 0.01 |
- |
in absence of bicarbonate |
678457 |
4.2.1.104 | 0.038 |
- |
purified recombinant mutant E94L, pH 7.7, 27°C |
716700 |
4.2.1.104 | 0.04 |
- |
purified recombinant mutant S117A, pH 7.7, 27°C |
716700 |
4.2.1.104 | 0.047 |
- |
complemented deficient Escherichia coli cells |
651632 |
4.2.1.104 | 0.22 |
- |
in the presence of 3 mM bicarbonate and 2 mM cyanate |
678457 |