EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   4.1.2.47 | -999 |
- |
cyanide-based high-throughput screening assay is developed. The assay is useful to detect activity and enantioselectivity of hydroxynitrile lyases theoretically towards any cyanohydrin substrate |
703181 |
| 4.1.2.47 | -999 |
- |
expressed in Saccharomyces cerevisiae or Pichia pastoris |
5159 |
| 4.1.2.47 | -999 |
- |
no specific activity is detected with recombinant wild type and mutant enzyme H103L when expressed in an Escherichia coli lysate (WakoPURE system) or as N-terminal His6-tagged enzyme in a RTS 100 wheat germ cell-free translation system |
713464 |
| 4.1.2.47 | -999 |
- |
screening assay for hydroxynitrile lyases and its application in high-throughput screening of Escherichia coli mutant libraries, semi-quantitative test where the rate of colour formation corresponds to the particular enzyme activity of the sample |
674985 |
| 4.1.2.47 | 0.1 |
- |
substrate: rac-mandelonitrile, pH 5.0, temperature not specified in the publication, mutant enzyme L121P |
747531 |
| 4.1.2.47 | 0.13 |
- |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme L121Y/L146M |
747531 |
| 4.1.2.47 | 0.13 |
- |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, wild-type enzyme |
747531 |
| 4.1.2.47 | 0.15 |
- |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme C81L |
747531 |
| 4.1.2.47 | 0.17 |
- |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme V106F |
747531 |
| 4.1.2.47 | 0.2 |
- |
substrate: rac-2-nitro-1-phenylethanol, pH 5.0, temperature not specified in the publication, mutant enzyme F125T/L146M |
747531 |
