EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.2.1.40 | -999 |
- |
activity analyzed at 4°C and 7°C, 100% activity arbitrarily established as the activity shown at 35°C, corresponds to 151 U/ml in Ralstonia pickettii, activity about 5% at 4°C and about 15% at 7°C determined |
679562 |
3.2.1.40 | -999 |
- |
activity measured under different conditions of pH and temperature, free cells and immobilized cells compared to defined conditions of large scale production of rhamnose, assay described, full degradation of 7.9 mM naringin by running the reactor at 1 ml per min at 50°C, optimal flow rates estimated, after four days substrate degradation capacity decreased by 10 to 15% |
679556 |
3.2.1.40 | -999 |
- |
assay in a reaction system containing 0.4 mM p-nitrophenyl-alpha-L-rhamnopyranoside as a substrate and 50 mM potassium phosphate buffer, resultant p-nitrophenol concentration determined spectrophotometrically |
677351 |
3.2.1.40 | -999 |
- |
assayed in a reaction system containing 0.4 mM p-nitrophenyl-alpha-L-rhamnopyranoside as a substrate and 50 mM potassium phosphate buffer, resultant p-nitrophenol concentration determined spectrophotometrically |
681462 |
3.2.1.40 | -999 |
- |
the isolate 005NJ retains 19% alpha-L-rhamnosidase activity at 7°C and 6% activity at 4°C, relative activity depends on carbon source, maximal specific activity obtained at concentrations of 0.5% (w/v) for L-rhamnose (967 U/mg) and apple pectin (382 U/mg) |
679562 |
3.2.1.40 | 0.324 |
- |
- |
136382 |
3.2.1.40 | 0.46 |
- |
hesperidin |
136398 |
3.2.1.40 | 1.5 |
- |
naringin |
136398 |
3.2.1.40 | 2.44 |
- |
purified enzyme |
666777 |
3.2.1.40 | 2.89 |
- |
purified enzyme |
666000 |