EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    3.2.1.133 | -999 |
- |
catalytic activity of wild-type LGMA and recombinant rLGMA tested in liquid and solid media, kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method, reaction at pH 6.5 for 13.5 h at 50°C, yields of 53.1% obtained |
681349 |
| 3.2.1.133 | -999 |
- |
kinetic mechanism for recombinant enzyme MAUS149 shown, determined by measuring the amount of reducing sugars released during incubation with starch using the dinitrosalicylic acid method, substrate concentrations ranging from 1 to 7.5 g/ml for starch and from 0.5 to 4 g/l for cyclodextrin and pullulan |
681861 |
| 3.2.1.133 | -999 |
- |
kinetic properties determined by amount of reducing sugars, assayed according to the dinitrosalicylic acid method |
680324 |
| 3.2.1.133 | -999 |
- |
kinetic properties determined in wild-type and mutant enzymes, relative activity indicated for several mutants from different lineages of DNA shuffling |
679745 |
| 3.2.1.133 | -999 |
- |
kinetic properties determined towards amylose and amylopectin in wild-type, double and triple mutant enzymes, bicinchonimate method, determination of reducing sugars |
678795 |
| 3.2.1.133 | -999 |
- |
specific activity of starch hydrolysis estimated for the high oligomer conformation is about 7 U/mg and for monomeric state about 110 U/mg |
678461 |
| 3.2.1.133 | 0.2 |
- |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant DM |
646805 |
| 3.2.1.133 | 0.3 |
- |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B74 |
646805 |
| 3.2.1.133 | 1 |
- |
pH 6.0, 60°C, beta-cyclodextrin as substrate, variant 4B78 |
646805 |
| 3.2.1.133 | 1.25 |
- |
pH 5.0, 90°C, substrate: maltotriose |
724097 |
