EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.1.3.10 | -999 |
- |
specific activities of recombinant enzymes similar to those of wild-type towards myo-inositol hexakisphosphate (i.e. phytate) and glucose 1-phosphate as substrates, entrapment in alginate beads, free enzyme looses less than 5% phosphatase activity within 2 h in 20 mM Tris-HCl buffer, pH 8.0, transfer limitation is responsible for the reduced reaction rate of the entrapped enzyme, complete conversion of myo-inositol hexakisphosphate into one single myo-inositol pentakisphosphate isomer, identified as D-myo-inositol(1,2,4,5,6)pentakisphosphate, shown to be feasible by using the enzyme-loaded alginate beads in batch operations |
691563 |
| 3.1.3.10 | 5.92 |
- |
purified recombinant His-tagged enzyme, pH and temperature not specified in the publication, substrate alpha-D-glucose 1-phosphate |
749654 |
| 3.1.3.10 | 30 |
- |
with nitrophenyl phosphate as substrate, purification procedure described |
134585 |
| 3.1.3.10 | 30 |
- |
with ribose 5-phosphate as substrate, purification procedure described |
134585 |
| 3.1.3.10 | 32 |
- |
substrate myo-inositol hexakisphosphate, 37°C, pH 5.0 |
663741 |
| 3.1.3.10 | 40 |
- |
purification procedure described |
134585 |
| 3.1.3.10 | 40 |
- |
substrate D-fructose 6-phosphate, 37°C, pH 5.0 |
663741 |
| 3.1.3.10 | 40 |
- |
with phosphoenolpyruvate as substrate |
134585 |
| 3.1.3.10 | 100 |
- |
purification procedure described |
134580 |
| 3.1.3.10 | 100 |
- |
with D-fructose 6-phosphate as substrate, purification procedure described |
134585 |
