EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
   3.1.1.25 | -999 |
- |
new enzymatic tests are developed that detect total PON1 levels, irrespective of high-density lipoprotein status and R/Q polymorphism, as well as the degree of catalytic stimulation and increased stability that follows tight binding of PON1 to HDLapoA-I. The tests are based on measuring total PON1 levels with a fluorogenic phosphotriester, measuring the lipolactonase activity with a chromogenic lactone, and assaying the chelator-mediated inactivation rate of the enzyme. The latter two are affected by tight binding of high-density lipoprotein and thereby derive the levels of the serum PON1-HDL complex. The new tests are demonstrated with a group of healthy individuals and show that the levels of PON1-HDL vary by a factor of 12 |
681264 |
| 3.1.1.25 | 0.0035 |
- |
pH 8.0, 25°C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.0036 |
- |
pH 8.0, 25°C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.0049 |
- |
pH 8.0, 25°C, substrate: DL-heptanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.011 |
- |
pH 8.0, 25°C, substrate: spironolactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.013 |
- |
pH 8.0, 25°C, substrate: canrenone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.0152 |
- |
substrate gamma-butyrolactone |
671559 |
| 3.1.1.25 | 0.0167 |
- |
pH 8.0, 25°C, substrate: DL-dodecanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.0255 |
- |
pH 8.0, 25°C, substrate: DL-tetradecanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
| 3.1.1.25 | 0.0268 |
- |
pH 8.0, 25°C, substrate: DL-3-oxo-hexanoyl-homoserine lactone, purified recombinant enzyme |
665960 |
