EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    2.7.8.13 | -999 |
- |
- |
662239 |
| 2.7.8.13 | -999 |
- |
0.42 units, using fluorescence enhancement assay, recombinant strain JM109 (pTrc99A), 11.9 units, recombinant strain JM109 (pBROC525), activity is expressed as increase in units of fluorescence emission per min at 30°C |
645340 |
| 2.7.8.13 | -999 |
- |
assay for MraY by using UDP-MurNAc-Nepsilon-dansylpentapeptide, a fluorescent derivative of the MraY nucleotide substrate. Two assay versions are developed, one consisting of the high-pressure liquid chromatography separation of the substrate and product and the other, without separation and adapted to the high-throughput format, taking advantage of the different fluorescent properties of the nucleotide and lipid I in the reaction medium |
660721 |
| 2.7.8.13 | -999 |
- |
specific activity is increased 2fold by induction with isopropyl-beta-D-thiogalactopyranoside in the late logarithmic phase |
645340 |
| 2.7.8.13 | -999 |
- |
specific activity of the enzyme increases 4fold by late-logarithmic phase |
645330 |
| 2.7.8.13 | 0.00002 |
- |
mutant H289R |
690996 |
| 2.7.8.13 | 0.00013 |
- |
mutant D99N |
690996 |
| 2.7.8.13 | 0.00024 |
- |
mutant D98N |
690996 |
| 2.7.8.13 | 0.000276 |
- |
specific activity of particulate enzyme from recombinant strain JM109 (pBROC525), in absence of exogenous lipid acceptor |
645340 |
| 2.7.8.13 | 0.00078 |
- |
mutant K116Q |
690996 |
