EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
2.5.1.20 | -999 |
- |
2.3 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for small rubber particles, 0.7 microM isopentenyl diphosphate incorporation/g dry rubber/4 h for purified and washed small rubber particles, almost no isopentenyl diphosphate incorporation/g dry rubber/4 h for washed and highly purified small rubber particles, in vitro, 50 mM phosphate buffer, pH 7.5, 40 mM 2-mercaptoethanol, 40 mM KF, 8 mM MgCl2 + substrates, 30°C, small rubber particle activity decreases with increasing dimethylallyl diphosphate concentration, dimethylallyl diphosphate slightly increases activity in washed small particles not in a dose-dependent manner |
709187 |
2.5.1.20 | 0.0000026 |
- |
2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h |
707862 |
2.5.1.20 | 0.000003 |
- |
2-methyl-1,3-butadiene incorporation assay with rubber particles, 100 mM Tris-HCl, pH 7.5, 30°C, 2 h |
707862 |
2.5.1.20 | 0.000695 |
- |
partially purified enzyme |
638730 |