EC Number   |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
|---|
    1.2.1.8 | -999 |
- |
- |
390352 |
| 1.2.1.8 | -999 |
- |
activities assayed in 400 ml of reaction mixture consisting of 20 mM Tris–HCl buffer, 0.5 mM NAD+, 5 mM DTT, and 100 ml purified protein elutes solution or crude bacterial extracts. The substrate betaine aldehyde is added to the reaction mixtures after preincubation for 10 min at 37°C to start the reactions. Enzyme activities are determined according to the absorbance at 340 nm (reflecting the consumption of NAD+). One unit of the activity is defined as 1 micromol/min of NAD+ consumption. The purified recombinant protein solution and crude bacterial extracts of the transformed cells both show high enzymatic activities. The activity is 2.53 nmol/ml and 2.06 nmol/ml, respectively, whereas crude bacterial extracts of untransformed Escherichia coli is 0.68 nmol/ml |
689664 |
| 1.2.1.8 | -999 |
- |
assay method |
390344 |
| 1.2.1.8 | -999 |
- |
Determination of aldehyde dehydrogenase activity of purified intact BADH2 and partial BADH2 using various aldehyde substrates (1 mM betaine aldehyde, 50 microM 4-aminobutyraldehyde, and 50 microM 3-aminopropionaldehyde). Enzymatic activities were spectrophotometrically assayed by A340 at pH 8.0 at intervals of 0, 10, 20, 30, and 60 min after the initiation of reactions. The intact BADH2 showed high betaine aldehyde dehydrogenase activity, with a rapid increase in A340 within the first 10 min after the enzymatic reaction. In addition, intact BADH2 also showed strong 4-aminobutyraldehyde and 3-aminopropionaldehyde dehydrogenase activities. |
689474 |
| 1.2.1.8 | -999 |
- |
quantification of 2-acetyl-1-pyrroline (2AP) and other metabolites in vivo |
763162 |
| 1.2.1.8 | 0.00014 |
- |
NaCl level 0%, wild type |
688198 |
| 1.2.1.8 | 0.00015 |
- |
NaCl level 1.5%, wild type |
688198 |
| 1.2.1.8 | 0.00017 |
- |
NaCl level 1.0%, wild type |
688198 |
| 1.2.1.8 | 0.00045 |
- |
NaCl level 0%, transgenic plant T3-5 |
688198 |
| 1.2.1.8 | 0.00078 |
- |
NaCl level 0%, transgenic plant T3-8 |
688198 |
