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Results 1 - 10 of 14 > >>
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Reference
-999
-
not detectable, 1 microM inositol 1,3,4,5-tetrakisphosphate and 1500 counts per minute of [3H]inositol 1,3,4,5-tetrakisphosphate, recombinant GST-IpgD expressed with chaperone IpgE (1-3 microg), reaction 37░C for 10 min in a 50 microl final volume containing 50 mM HEPES pH 7.4, 0.1% bovine serum albumin, 2 mM MgCl2, and 0.033% beta-mercaptoethanol
0.0000003
-
approx., C462A mutant, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000005
-
approx., R468A mutant, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000007
-
approx., GST, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000011
-
approx., DELTA1-351 mutant, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000015
-
approx., DELTA352-563 mutant, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000032
-
approx., DELTA118-142 mutant, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0000063
-
approx., SigD, expressed in Escherichia coli DH5alpha, GST-tagged, malachite-green phosphatase assay (phosphatidylinositol 3,4,5-trisphosphate)
0.0003
-
10 microM concentration of inositol 1,4,5-trisphosphate and 1500 counts per minute of [3H]inositol 1,4,5-trisphosphate, recombinant GST-IpgD expressed with chaperone IpgE (1-3 microg), reaction 37░C for 10 min in a 50 microl final volume containing 50 mM HEPES pH 7.4, 0.1% bovine serum albumin, 2 mM MgCl2, and 0.033% b-mercaptoethanol, similar results when expressed without IpgE
0.0058
-
50 microl of recombinant GST-IpgD expressed with chaperone IpgE (1-3 microg) in 50 mM Tris pH 7.5 containing 50 mM glutathione and 100 mM NaCl , 37░C, 15 min, reaction mixture contained 10 microM [32P] phosphatidylinositol 3-phosphate and 20 microM phosphatidylserine, similar results when expressed without IpgE
Results 1 - 10 of 14 > >>