EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.1.3.21 | -999 |
- |
changes in glucose, glycerol and 2,3-butanediol determined in engineered bacteria, new metabolic pathway of glucose via glycerol to 1,3-propanediol constructed, effect of pH on production of 1,3-propanediol, effect of dihydroxyacetone and pH, comparison of enzyme activities growing on different substrates, successful introduction of a glycerol pathway of yeast into Klebsiella pneumoniae |
687860 |
3.1.3.21 | -999 |
- |
L-glycerol 3-phosphate (L-G3P) production via microbial fermentation, elimination of both GPP1 and GPP2 isoenzymes required, small-scale and large-scale batch fermentation, intracellular accumulation of L-glycerol 3-phosphate reaches high levels of about 200 mM, thereafter accumulation declines, extracellular L-glycerol 3-phosphate concentration increases throughout fermentation, most of the total L-glycerol 3-phosphate outside the cells, specific elimination of enzyme activity reveals considerable glycerol formation, unspecific dephosphorylation as a mechanism to relieve cells of intracellular L-glycerol 3-phosphate accumulation, final product titer of about 325 mg total L-glycerol 3-phosphate per litre of fermentation broth |
685707 |
3.1.3.21 | 0.35 |
0.75 |
dependency on subcellular fraction |
81027 |
3.1.3.21 | 0.63 |
- |
- |
81031 |
3.1.3.21 | 0.8 |
- |
- |
81033 |
3.1.3.21 | 6.1 |
- |
- |
81030 |
3.1.3.21 | 46 |
- |
enzyme Gpp2p |
81025 |
3.1.3.21 | 49 |
- |
enzyme Gpp1p |
81025 |