   4.2.2.7 | cell culture |
optimization of enzyme production, simple carbon sources, including sucrose, lactose, maltose, and complex carbon sources such as starch and cellulose are able to support growth of the organism, but enzyme can be produced only in the presence of maltose, sorbitol, mannitol, arabinose, and mannose. Phosphate inhibits enzyme production and cell growth, while heparin inhibits cell growth but is required for enzyme induction, overview. Heparinase production in optimized medium is highest, i.e., 75.4 units, when the pH of the medium is kept at pH 6.5, inoculum size of 0.5-1.0% of spore suspension, and for an incubation temperature of 30°C. Growth profile, heparin utilization, and heparinase production in optimized medium, overview |
693981 |
