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Renatured (Commentary)
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2.8.1.3
enzyme loses catalytic activity in 6 M guanidinium hydrochloride, renaturation after a short period of time by dialysis is rapid and complete. Renaturation after 10 weeks results in only partial restoration of enzyme activity. Full renaturation can be obtained by adding thioglycolate or 2-mercaptoethanol during denaturation. Renaturation rate is temperature dependent
645574
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