1.1.1.82 | solubilization and refolding of recombinant MDH from Escherichia coli inclusion bodies, the enzyme is dissolved in 6 M guanidine-HCl and gradually refolded to the active enzyme through dilution of the denaturant, conditions: refolding buffer 1.5 L of 0.1 M Tris-HCl, pH 7.5, containing 2 mM EDTA and 0.4 M L-arginine, incubation for 36 h at 4°C |
696461 |