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EC Number Renatured (Commentary) Reference
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198- 762321
Show all pathways known for 1.1.1.198Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.198recombinant enzyme from Escherichia coli inclusion bodies, solubilized in 25 ml of 0.1 M potassium phosphate buffer, pH 7.0, containing 6 M urea, 10 mM DTT, and 1 mM EDTA, for 2 h at room temperature stirred. Refolding by dissolution in 8 M urea and dialysis against 10 mM potassium phosphate buffer in the presence of 10% glycerol results in an inactive enzyme 739915
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