EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.2.1.167 | 2.58 |
- |
estrone 3-(beta-D-glucuronide) |
recombinant His-tagged enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 3.15 |
- |
wogonoside |
recombinant His-tagged enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 4.2 |
- |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate |
pH 6.5, 28°C, mutant enzyme E329A |
655984 |
3.2.1.167 | 10.6 |
- |
Baicalin |
recombinant His-tagged enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 13.1 |
- |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate |
pH 6.5, 28°C, mutant enzyme E212A |
655984 |
3.2.1.167 | 14 |
- |
Baicalin |
native enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 28.5 |
- |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate |
pH 6.5, 28°C, mutant enzyme Y281A |
655984 |
3.2.1.167 | 204 |
- |
4-nitrophenyl beta-D-glucuronide |
recombinant His-tagged enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 348 |
- |
4-nitrophenyl beta-D-glucuronide |
native enzyme, pH 5.0, 37°C |
731207 |
3.2.1.167 | 52810 |
- |
5,6,7-trihydroxyflavone 7-O-beta-D-glucoronate |
pH 6.5, 28°C, wild-type enzyme |
655984 |