EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
3.1.3.75 | 1.06 |
- |
Phosphoethanolamine |
50 mM Hepes/NaOH buffer, pH 7.0, 37°C |
714360 |
3.1.3.75 | 2.34 |
- |
phosphocholine |
50 mM Hepes/NaOH buffer, pH 7.0, 37°C |
714360 |
3.1.3.75 | 13.3 |
- |
4-nitrophenyl phosphate |
purified recombinant mutant Y82A/Y83A/Y84A, pH 5.0, 37°C |
714357 |
3.1.3.75 | 21.4 |
- |
4-nitrophenyl phosphate |
purified recombinant mutant E42A, pH 5.0, 37°C |
714357 |
3.1.3.75 | 24 |
- |
p-nitrophenyl phosphate |
C94A mutant protein |
706604 |
3.1.3.75 | 29 |
- |
p-nitrophenyl phosphate |
wild-type protein |
706604 |
3.1.3.75 | 44 |
- |
p-nitrophenyl phosphate |
C87A mutant protein |
706604 |
3.1.3.75 | 57.2 |
- |
4-nitrophenyl phosphate |
purified recombinant wild-type enzyme, pH 5.0, 37°C |
714357 |
3.1.3.75 | 141 |
- |
4-nitrophenyl phosphate |
purified recombinant mutant E43A, pH 5.0, 37°C |
714357 |