EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    2.5.1.54 | 0.069 |
- |
D-erythrose 4-phosphate |
pH 7.5, 30°C |
723700 |
| 2.5.1.54 | 0.21 |
- |
D-erythrose 4-phosphate |
pH 7.5, 60°C, mutant enzyme I181D |
725725 |
| 2.5.1.54 | 0.22 |
- |
phosphoenolpyruvate |
pH 7.5, 60°C, mutant enzyme I181D |
725725 |
| 2.5.1.54 | 0.35 |
- |
phosphoenolpyruvate |
pH 7.5, 30°C |
723700 |
| 2.5.1.54 | 49 |
- |
phosphoenolpyruvate |
pH 7.5, 60°C, wild-type enzyme |
725725 |
| 2.5.1.54 | 60 |
- |
D-erythrose 4-phosphate |
pH 7.5, 60°C, wild-type enzyme |
725725 |
| 2.5.1.54 | 84 |
- |
phosphoenolpyruvate |
results within factor two of this otherwise published value, 30°C, 50 mM BTP (1,3-bis[tris(hydroxymethyl)methylamino]propane), pH 7.5 with 100 microM MnCl2, 240 microM D-erythrose-4-phosphate (saturating), less than 10 microM phosphoenolpyruvate, no influence of chorismate mutase concentration on activity |
703449 |
| 2.5.1.54 | 360 |
- |
phosphoenolpyruvate |
pH 7.3, temperature not specified in the publication, mutant H29S/S31H |
738228 |
| 2.5.1.54 | 370 |
- |
D-erythrose 4-phosphate |
pH 6.8, 30°C, mutant R126S |
739599 |
| 2.5.1.54 | 430 |
- |
D-erythrose 4-phosphate |
pH 7.3, temperature not specified in the publication, mutant S31G |
738228 |
