EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.8.1.14 | 0.00027 |
- |
NADH |
wild type enzyme, at 25°C |
685242 |
1.8.1.14 | 0.0093 |
- |
NADPH |
wild type enzyme, at 25°C |
685242 |
1.8.1.14 | 2.1 |
- |
methyl methanethiolsulfonate |
mutant enzyme Y419F, at 23°C, pH not specified in the publication |
724355 |
1.8.1.14 | 27 |
- |
CoA-disulfide |
pH 8.1, 50°C, cofactor: NADPH |
744312 |
1.8.1.14 | 54 |
- |
CoA-disulfide |
pH 8.1, 50°C, cofactor: NADH |
744312 |
1.8.1.14 | 82 |
- |
methyl methanethiolsulfonate |
wild type enzyme, at 23°C, pH not specified in the publication |
724355 |
1.8.1.14 | 220 |
- |
methyl methanethiolsulfonate |
mutant enzyme Y361F, at 23°C, pH not specified in the publication |
724355 |
1.8.1.14 | 9000 |
- |
CoA-disulfide |
wild type enzyme, at 23°C, pH not specified in the publication |
724355 |