EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.5.1.33 | 2.77 |
- |
7,8-dihydromonapterin |
recombinant mutant Y161A, pH 6.0, 25°C |
765008 |
1.5.1.33 | 4.72 |
- |
7,8-dihydromonapterin |
recombinant mutant R24A, pH 6.0, 25°C |
765008 |
1.5.1.33 | 6 |
- |
7,8-dihydromonapterin |
recombinant mutant Y161F/Y163F, pH 6.0, 25°C |
765008 |
1.5.1.33 | 19.75 |
- |
7,8-dihydromonapterin |
recombinant mutant Y163F, pH 6.0, 25°C |
765008 |
1.5.1.33 | 21 |
- |
quinonoid 6,7-dimethyl-7,8-dihydropterin |
in 50 mM HEPES buffer, pH 7.4, at 25°C |
725417 |
1.5.1.33 | 25.24 |
- |
7,8-dihydroneopterin |
recombinant wild-type enzyme, pH 6.0, 25°C |
765008 |
1.5.1.33 | 26 |
- |
quinonoid 6,7-dimethyl-7,8-dihydropterin |
in 50 mM HEPES buffer, pH 7.4, at 25°C |
725417 |
1.5.1.33 | 32.26 |
- |
7,8-dihydromonapterin |
recombinant wild-type enzyme, pH 6.0, 25°C |
765008 |
1.5.1.33 | 135.45 |
- |
7,8-dihydrobiopterin |
recombinant wild-type enzyme, pH 6.0, 25°C |
765008 |
1.5.1.33 | 160 |
- |
quinonoid dihydrobiopterin |
in 50 mM HEPES buffer, pH 7.4, at 25°C |
725417 |