EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.16.1.9 | 0.02 |
- |
Fe(III)-aerobactin |
wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.032 |
- |
Fe(III)-enterobactin |
mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.076 |
- |
Fe(III)-enterobactin |
mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.091 |
- |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 |
mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.12 |
- |
Fe(III)-EDTA |
in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C |
721789 |
1.16.1.9 | 0.132 |
- |
Fe(III)-(N-2,3-dihydroxybenzoyl-L-serine)3 |
mutant enzyme R130A in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.139 |
- |
Fe(III)-dicitrate |
mutant enzyme R130A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 0.271 |
- |
Fe(III)-dicitrate |
mutant enzyme K55A, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 1.268 |
- |
Fe(III)-vibriobactin |
wild type enzyme, in 50 mM Tris-HCl (pH 8.0) and 100 mM NaCl at 25°C |
721629 |
1.16.1.9 | 1.59 |
- |
Fe(III)-citrate |
in the absence of FAD, in 50 mM sodium phosphate buffer (pH 7.0) containing 0.2 mM NADPH at 25°C |
721789 |