EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.14.14.151 | 60 |
- |
5-epiaristolochene |
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 80 |
- |
5-epiaristolochene |
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 150 |
- |
(-)-vetispiradiene |
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 260 |
- |
valencene |
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 280 |
- |
5-epiaristolochene |
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 300 |
- |
valencene |
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 610 |
- |
(-)-vetispiradiene |
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 650 |
- |
5-epiaristolochene |
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 740 |
- |
valencene |
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |
1.14.14.151 | 1310 |
- |
valencene |
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30°C |
712360 |