EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
---|
1.1.1.343 | 1.2 |
- |
NADP+ |
pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme |
761070 |
1.1.1.343 | 2 |
- |
NADP+ |
pH 7.5, temperature not specified in the publication, membrane-purified enzyme |
761070 |
1.1.1.343 | 3.4 |
- |
NAD+ |
mutant enzyme N32D/R33L/T34S, in 100 mM HEPES (pH 7.5), at 70°C |
741428 |
1.1.1.343 | 3.9 |
- |
6-phospho-D-gluconate |
pH 7.5, temperature not specified in the publication, cytoplasm-purified enzyme |
761070 |
1.1.1.343 | 5.4 |
- |
NAD+ |
wild type enzyme, in 100 mM HEPES (pH 7.5), at 70°C |
741428 |
1.1.1.343 | 7.3 |
- |
NAD+ |
mutant enzyme N32D, in 100 mM HEPES (pH 7.5), at 70°C |
741428 |
1.1.1.343 | 8.3 |
- |
NAD+ |
mutant enzyme N32D/R33I/T34I, in 100 mM HEPES (pH 7.5), at 70°C |
741428 |
1.1.1.343 | 9 |
- |
NAD+ |
wild type enzyme, in 100 mM HEPES (pH 7.5), at 50°C |
741424 |
1.1.1.343 | 14.8 |
- |
NAD+ |
mutant enzyme A30C/R31I/T32K, in 100 mM HEPES (pH 7.5), at 50°C |
741424 |
1.1.1.343 | 15 |
- |
NAD+ |
mutant enzyme R31T, in 100 mM HEPES (pH 7.5), at 50°C |
741424 |