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EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Commentary	Reference
2.4.2.17	0.21	-	ATP	truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C	758757
2.4.2.17	0.214	-	ATP	at pH 8.5 and 10°C	758806
2.4.2.17	0.32	-	ADP	subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C	776638
2.4.2.17	0.32	-	ATP	at pH 8.5 and 15°C	758806
2.4.2.17	0.4	-	5-phospho-alpha-D-ribose 1-diphosphate	subunit HisGs, with ADP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C	776638
2.4.2.17	0.422	-	ATP	at pH 8.5 and 20°C	758806
2.4.2.17	0.46	-	ATP	subunit HisGs, in the presence of Mg2+, at pH 7.5 and 25°C	776638
2.4.2.17	0.64	-	5-phospho-alpha-D-ribose 1-diphosphate	subunit HisGs, with ATP as cosubstrate, in the presence of Mg2+, at pH 7.5 and 25°C	776638
2.4.2.17	0.682	-	ATP	at pH 8.5 and 25°C	758806
2.4.2.17	0.75	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 8.5 and 10°C	758806

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Release 2026.1 (March 2026)