EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference  |
|---|
   1.1.99.29 | 5.5 |
- |
D-galactose |
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 7.1 |
- |
D-glucose |
mutant enzyme H103Y, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 44.2 |
- |
D-galactose |
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 47.4 |
- |
1,4-benzoquinone |
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C |
741303 |
| 1.1.99.29 | 54.8 |
- |
D-glucose |
wild type enzyme, with ferrocenium ion as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 140 |
- |
2,6-dichlorophenolindophenol |
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 4.0 and 30°C |
741303 |
| 1.1.99.29 | 260 |
- |
ferrocenium ion |
mutant enzyme H103Y, with D-glucose as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 290.7 |
- |
2,6-dichlorophenolindophenol |
wild type enzyme, with D-glucose as cosubstrate, at pH 4.0 and 30°C |
741303 |
| 1.1.99.29 | 812.5 |
- |
ferrocenium ion |
wild type enzyme, with D-glucose as cosubstrate, at pH 8.5 and 30°C |
741303 |
| 1.1.99.29 | 0.1 |
- |
D-mannose |
with ferrocenium ion as electron acceptor, in 100 mM sodium phosphate pH 7.5, at 30°C |
741362 |
