EC Number   |
General Information |
Reference |
|---|
   4.2.3.13 | evolution |
the CAD is encoded by a gene family, which is divided into two subfamilies CAD1-A and CAD1-C (Cdn1-C1, Cdn1-C14, Cdn1-A and Cdn1-C3), based on sequence similarities. The diploid genome of Gossypium arboreum contains about six members of CAD1- C and a single copy of CAD1-A |
748919 |
| 4.2.3.13 | malfunction |
if W279 in delta-cadinene synthase is replaced with various smaller amino acids, the ratio of alcohol versus hydrocarbon product is directly proportional to the van der Waals volume of the amino acid side chain. Mutant DCS-W279A is a catalytically highly efficient germacradien-4-ol synthase that produces predominantly germacradien-4-ol in addition to 11% delta-cadinene |
747551 |
| 4.2.3.13 | metabolism |
possible mechanism of sesquiterpene synthases from Piper nigrum. The reaction pathway is based on 1,10-ring closure pathway including enzyme PnCO/CDS |
746924 |
| 4.2.3.13 | more |
catalytic mechanisms of (+)-delta-cadinene synthase (DCS) and germacradien-4-ol synthase (GdolS) catalysed conversions of (2E,6E)-farnesyl diphosphate to of (+)-delta-cadinene and germacradien-4-ol, analysis of the mechanism by which sesquiterpene synthases discriminate between deprotonation and reaction with a nucleophilic water molecule, molecular dynamics simulations, overview. Enzyme active site structure with bound Mg2+ ions |
747551 |
| 4.2.3.13 | more |
PnTPS2-transformed yeast cells accumulate delta-cadinol as a major product (91%) and smaller amounts of alpha-cadinene (2%) and delta-cadinene (7%). Recombinant expression of Piper nigrum germacrene D synthase (PnGDS) in yeast yields increased beta-cadinene and alpha-copaene contents |
746924 |
| 4.2.3.13 | physiological function |
enzyme DCS from Gossypium arboreum produces (+)-delta-cadinene in the first committed step of the biosynthetic pathway to the phytoalexin gossypol |
747551 |
| 4.2.3.13 | physiological function |
in cotton, (+)-delta-cadinene synthase (CAD) catalyses the cyclization of farnesyl diphosphate to (+)-delta-cadinene via a nerolidyl diphosphate intermediate and is the first step in the biosynthesis of gossypol and its precursor terpene aldehyde |
748919 |
| 4.2.3.13 | physiological function |
in cotton, (+)-delta-cadinene synthase (CAD) catalyses the cyclization of farnesyl diphosphate to (+)-delta-cadinene via a nerolidyl diphosphate intermediate and is the first step in the biosynthesis of gossypol and its precursor terpene aldehyde. Increased enzyme expression due to jasmonic acid treatment or plant growth-promoting rhizobacteria (PGPR) treatment leads to increased levels of gossypol in plants. Cotton pathogen Spodoptera exigua shows reduced larval feeding and development on PGPR-treated plants |
748919 |
| 4.2.3.13 | physiological function |
the enzyme distributes to systemic resistance in cotton against beet armyworm Spodoptera exigua |
748919 |
| 4.2.3.13 | physiological function |
the enzyme is involved in biosynthesis of beta-copaene and beta-cadinene (EC 4.2.3.13). In the unripe fruit, thirteen sesquiterpenes including beta-caryophyllene, delta-elemene, alpha-copaene, cubebene, alpha-humulene, delta-cadinol, and gamma- and delta-cadinenes are found. beta-Caryophyllene is the most abundant compound among the observed sesquiterpenes (65.8% of the total), followed by copaene (8.3%). During fruit ripening, the contents of the sesquiterpenes derived from germacrene D, such as cubebene, copaene, cubeol, and germacrene D-4?-ol, are progressively declined, most likely due to diminished total germacrene D synthase activity |
746924 |
