EC Number   |
General Information |
Reference |
|---|
   3.5.1.115 | evolution |
some Gram-positive bacteria, such as members of Corynebacterium, Mycobacterium, Rhodococcus and Streptomyces, cannot produce GSH but instead synthesize its functional equivalent, mycothiol |
-, 735129 |
| 3.5.1.115 | malfunction |
mutants lacking the enzyme and mycothiol are more susceptible to alkylating agents and oxidants, e.g. monobromobimane, iodoacetamide, N-ethylmaleimide, 1-chloro-2,4-dinitrobenzene, and methyllglyoxal, or to some macrolides and beta-lactams, e.g. rifamycin S, than the wild-type enzyme. Heavy metal ions, including Cd2+, Ni2+, Cr2+ and Cu2+, markedly inhibit growth of the mca mutant relative to the wild type strain |
-, 735129 |
| 3.5.1.115 | malfunction |
the Ami37 mutation in mca renders the mutant strain more susceptible to electrophilic toxins, such as N-ethylmalemide, iodoacetamide, and chlorodinitrobenzene, and to several oxidants, such as menadione and plumbagin. The mca mutant is also more susceptible to the antituberculous antibiotic streptomycin |
-, 725235 |
| 3.5.1.115 | metabolism |
mycothiol S-conjugate amidase plays an important role in the detoxification of alkylating agents and antibiotics |
724260 |
| 3.5.1.115 | metabolism |
mycothiol serves as the primary reducing agent in Mycobacterium species, and is also a cofactor for the detoxification of xenobiotics. The enzyme catalyzes the cleavage of mycothiol-S-conjugates to form a mercapturic acid, which is excreted from the mycobacterium |
752920 |
| 3.5.1.115 | more |
residues Asp14, Tyr137, His139 and Asp141 are important for enzyme activity |
-, 735129 |
| 3.5.1.115 | physiological function |
mycothiol S-conjugate amidase is a key enzyme involved in MSH-dependent detoxification. In detoxification process, mycothiol directly reacts with electrophilic compounds by its thiol moiety, forming MSH S-conjugates, regulation mechanism, potential roles of the enzyme in resistance to alkylating agents and oxidants and in the survival of Corynebacterium glutamicum by coping with multiple stresses and detoxifying toxins, overview |
-, 735129 |
