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Results 1 - 10 of 14 > >>
EC Number General Information Commentary Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113evolution comparison of the Flaviviridae NS3 proteases 754554
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113evolution the enzyme is well conserved among the Flaviviridae 732796
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113malfunction altering either of the autocleavage sites (Leu192/Met193 and Leu159/Lys160) by Leu deletion or mutation greatly inhibits RNA replication in a CSFV replicon system and results in a loss of genome RNA infectivity 754554
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113malfunction antibody 3F10 disrupts the interaction between the cNS2B cofactor region and NS3 protease in vitro and reduces viral replication in HEK-293 cells 717403
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113malfunction deletion of Leu1781 results in enzyme inactivation 732415
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113malfunction the gain of function mutation 3/V132A as well as the mutations in the NS4A-kink region expose the TEV cleavage site suggesting a destabilization of the NS3/4A-kink interaction. Single mutations at the NS3/4A-kink interface allow for polyprotein processing and RNA replication. The gain of function mutation 3/V132A can be functionally substituted by the single mutations 4A/L45A or 4A/Y47A in NS2-3-independent virion morphogenesis. The replication-deficient NS3/4A double mutant 4A/L45-Y47-AA is functional in viral packaging when supplied in trans. Analysis of NS3 and NS2 mutations combined, overview 755207
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113metabolism internal cleavage within the NS3 moiety is a common process that further extends the functional repertoires of the multifunctional NS2-3 or NS3 and represents another level of the complex polyprotein processing of Flaviviridae 732415
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113metabolism upon infection of the host cell the viral RNA genome is translated into a polyprotein that is processed by cellular and viral proteases into the mature structural (SP) and nonstructural (NS) proteins. For members of the genus Pestivirus the array in the polyprotein is the following: NH2-Npro (N-terminal autoprotease), C (capsid protein, core), Erns (envelope protein RNase secreted), E1, E2, p7, NS2-3 (NS2 and NS3), NS4A, NS4B, NS5A, NS5B-COOH. The N-terminal autoprotease Npro generates its own C-terminus and thereby the N-terminus of the capsid protein core (C). Further cleavages releasing the structural proteins C, Erns, E1 and E2 as well as p7 are mediated by proteases residing in the endoplasmatic reticulum (ER). The cleavage between NS2 and NS3 is catalyzed by an autoprotease in NS2. The activity of the NS2 protease is temporally regulated by a cellular cofactor leading to significant amounts of uncleaved NS2-3 in pestivirus infected cells. The cleavages downstream of NS3, NS4A, NS4B and NS5A are catalyzed by the serine protease domain of NS3 which requires NS4A as cofactor for full proteolytic activity and is termed NS3-4A protease 755207
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113more structure-function analysis suggests that NS3/4A can adopt two different conformations in the infected cell, a closed form that is used in RNA replication complexes and a more open conformation functional in viral assembly. Furthermore, the NS2-3/4A complex, required for virion assembly of prototype pestiviruses, displays a similar open conformation. CSFV NS3/4A complex crystal structure analysis, overview 755207
Display the word mapDisplay the reaction diagram Show all sequences 3.4.21.113more the catalytic triad is formed by residues H69, D97, and S163. The intramolecular interface between protease and helicase in CSFV NS3 is featured by three clusters of interactions, overview 754554
Results 1 - 10 of 14 > >>