EC Number   |
General Information |
Reference |
|---|
   2.5.1.75 | evolution |
while absent in eukaryotes, some facultative bacteria use a non-heme diiron enzyme [MiaE, 2-methylthio-N6-isopentenyladenosine(37)-tRNA monooxygenase] to catalyze the O2-dependent hydroxylation of ms2i6A37 to produce 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37), comparison of MiaA and MiaE catalytic reactions, overview |
-, 737708 |
| 2.5.1.75 | malfunction |
knockdown of Trit1 reduces expression of selenoproteins. Trit1 proteins carrying the D55G or T32A mutations exhibit significantly reduced specific activity of 50% and 10%of wild-type respectively. Overexpression of TRIT1 does not increase selenoprotein expression in HepG2 cells |
737635 |
| 2.5.1.75 | metabolism |
the enzyme is involved in the hypermodification enzyme pathway for 2-methylthio-N6-(4-hydroxyisopentenyl)-adenosine (ms2io6A37) synthesis. within the anticodon stem loop of tRNA, nucleotide modifications are frequently observed at position 34 (the wobble position) or position 37 (3' adjacent to the anticodon), nearly all eukaryotic and bacterial tRNAs that read codons starting with U are modified by the enzymes MiaA and MiaB and contain the ms2i6A modification |
-, 737708 |
| 2.5.1.75 | physiological function |
enzyme MiaA catalyzes the nucleophilic substitution of the dimethylallyl group from dimethylallyl diphosphate (DMAPP) to the exocyclic 6-amino nitrogen of A37 (N6) to yield N6-isopentenyladenosine (i6A37) with release of inorganic diphosphate |
-, 737708 |
| 2.5.1.75 | physiological function |
mammalian Trit1 is a tRNA([Ser]Sec)-isopentenyl transferase required for full selenoprotein expression, full expression of selenoproteins requires modification of tRNA[Ser]Sec, including N6-isopentenylation of base A37 |
737635 |
| 2.5.1.75 | physiological function |
Trit1 exhibits substrate-specific restriction against Schizosaccharomyces pombe cytosolic tRNATrpCCA-A37-A38. Position 32 of tRNATrpCCA may be a conditional determinant for substrate-specific i6A37 modification by Trit1 |
760010 |
