EC Number   |
General Information |
Reference |
|---|
   2.3.1.75 | evolution |
among six possible predicted Euglena WSD orthologues, gene expression analysis and in vivo evaluation for enzyme activity with yeast expressing individual recombinant WSDs indicates that two of them, EgWSD2 and EgWSD5, predominantly function as wax ester synthase. The six WSD isoforms in Euglena gracilis all contain WES and DUF1298 motifs |
758397 |
| 2.3.1.75 | evolution |
distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively |
-, 756245 |
| 2.3.1.75 | evolution |
distribution of putative WS/DGAT in Streptomyces genomes, overview |
755845 |
| 2.3.1.75 | malfunction |
remarkably reduced wax ester contents in EgWSD2/5-double knockdown Euglena gracilis cells treated with anaerobic conditions. The decreased ability to produce wax ester does not affect adaptation of Euglena gracilis to anaerobiosis. Lipid profile analysis suggests an allocation of metabolites to other compounds including triacylglycerol instead of wax esters, lipid profile analyses of EgWSD2/5 double-knockout cells, effect of EgWSD gene silencing on wax ester production, overview. The relative amount of wax esters, including C28, is significantly low in EgWSD2/5 double-KD cells, whereas that of fatty acids does not differ between the silenced cells and mock control cells |
758397 |
| 2.3.1.75 | malfunction |
the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain |
755845 |
| 2.3.1.75 | metabolism |
the enzyme plays a pivotal role in wax ester synthesis |
758397 |
| 2.3.1.75 | more |
quantitative expression analysis of WS and WSD genes in response to aerobic and anaerobic conditions |
758397 |
| 2.3.1.75 | physiological function |
enzyme AtfG25 has an important, but not exclusive, role in triacylglycerol (TAG) biosynthesis in the Streptomyces sp. G25 isolate, suggesting the presence of alternative metabolic pathways for lipid accumulation |
755845 |
| 2.3.1.75 | physiological function |
pivotal role of both EgWSD2 and EgWSD5 in wax ester synthesis |
758397 |
| 2.3.1.75 | physiological function |
two bifunctional enzymes, TrWSD4 and TrWSD5, from the marine protist Thraustochytrium roseum show wax ester synthase/acyl-CoA:diacylglycerol acyltransferase activity catalyzing wax ester and triacylglycerol synthesis. The WS/DGAT shows both in vitro WS and DGAT activity, it is characterized as unspecific acyltransferase accepting a broad range of acyl-CoAs and fatty alcohols as substrates for WS activity but displaying substrate preference for medium-chain acyl-CoAs. In vivo characterization shows that the WS/DGAT predominantly functions as wax synthase |
-, 756245 |
