EC Number   |
General Information |
Reference |
|---|
   2.1.1.274 | evolution |
the enzyme belongs to SABATH family, a class of O-methyltransferases and N-methyltransferases |
736189 |
| 2.1.1.274 | evolution |
the enzyme belongs to the protein family of SABATH methyltransferases, ten genes encode isozymes PaSABATH1-10. Five of the PaSABATH isozymes (PaSABATH3, PaSABATH6, PaSABATH7, PaSABATH8, and PaSABATH9) do not show activity with any of the four substrates, i.e. indole-3-acetic acid, jasmonic acid, giberellic acid A3, and salicylic acid, the other five of the PaSABATHs each show activity with one or more of the four substrates. PaSABATH1 has the highest level of specific activity with indole-3-acetic acid and is renamed as PaIAMT (EC 2.1.1.275). PaSABATH2 has the highest level of specific activity with salicylic acid and is designated as PaSAMT (EC 2.1.1.274). For comparison, PaSAMT is also assayed with two compounds of similar structure benzoic acid and anthranilic acid (cf. EC 2.1.1.273). While PaSAMT has no activity with anthranilic acid, its activity with benzoic acid is approximately 8% of that with salicylic acid. PaSABATH4, PaSABATH5 and PaSABATH10 show the highest level of specific activity with jasmonic acid and are renamed PaJAMT1, PaJAMT2, and PaJAMT3, respectively (EC 2.1.1.141). Their products are confirmed to be methyljasmonate |
757941 |
| 2.1.1.274 | evolution |
the enzyme belongs to the SABATH family, phylogenetic analysis and tree, detailed overview. Twenty-eight Populus SABATH genes are divided into three classes with distinct divergences in their gene structure, expression responses to abiotic stressors and enzymatic properties of encoded proteins. Populus class I SABATH proteins convert indole-3-acetic acid (IAA) to methyl-IAA, class II SABATH proteins convert benzoic acid (BA) and salicylic acid (SA) to methyl-BA and methyl-SA, while class III SABATH proteins convert farnesoic acid (FA) to methyl-FA. For Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. Of the Populus SABATH class II proteins, PtSABATH4 and 24 show the highest activity towards SA and BA, respectively |
757984 |
| 2.1.1.274 | malfunction |
a knockout mutant fails to accumulate methyl salicylate following pathogen infection. These plants also fail to accumulate salicylate or its glucoside in the uninoculated leaves and do not develop systemic acquired resistance. However, the mutant exhibits normal levels of effector-triggered immunity and pathogen-associated molecular pattern-triggered immunity to Pseudomonas syringae and Hyaloperonospora arabidopsidis |
726013 |
| 2.1.1.274 | malfunction |
AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview |
-, 757709 |
| 2.1.1.274 | malfunction |
basal salicylic acid (SA) levels in Arabidopsis thaliana plants that constitutively overexpress PbBSMT compared with those in Arabidopsis wild-type Col-0 are reduced approximately 80% versus only a 50% reduction in plants overexpressing AtBSMT1. PbBSMT-overexpressing plants are more susceptible to Plasmodiophora brassicae than wild-type plants, they also are partially compromised in nonhost resistance to Albugo candida. In contrast, AtBSMT1-overexpressing plants are not more susceptible than wild-type to either Plasmodiophora brassicae or Albugo candida. Furthermore, transgenic Arabidopsis thaliana and Nicotiana tabacum plants overexpressing PbBSMT exhibit increased susceptibility to virulent Pseudomonas syringae pv. tomato DC3000 and virulent Pseudomonas syringae pv. tabaci, respectively. Gene-mediated resistance to DC3000/AvrRpt2 and tobacco mosaic virus (TMV) is also compromised in Arabidopsis thaliana and Nicotiana tabacum cv. Xanthi-nc plants overexpressing PbBSMT, respectively. Transient expression of PbBSMT or AtBSMT1 in lower leaves of Nicotiana tabacum cv. Xanthi-nc results in systemic acquired resistance (SAR)-like enhanced resistance to TMV in the distal systemic leaves. The development of a PbBSMT-mediated SAR-like phenotype is also dependent on the MeSA esterase activity of NtSABP2 in the systemic leaves. Phenotypes, overview |
757709 |
| 2.1.1.274 | malfunction |
for Populus class II SABATH proteins, both forward and reverse mutagenesis studies show that a single amino acid switch between PtSABATH4 and PtSABATH24 results in substrate switch. The mutation of Met156 to His results in a switch from a preference for salicylic acid (SA) over benzoic acid (BA) in wild-type PtSABATH4 to a preference for BA over SA in the M156H mutant. The mutation of His157 of PtSABATH24 (EC 2.1.1.273) to a methionine residue also results in a switch from a preference for BA over SA in wild-type PtSABATH24 to a preference for SA over BA in the H157M mutant. The PtSABATH4 mutation M314V results in decreased enzymatic activities towards both the substrates salicylic acid (SA) over benzoic acid (BA), but not in a substrate switch |
757984 |
| 2.1.1.274 | malfunction |
overexpression of LcSAMT gene markedly enhances the methylsalicylate (MeSA) content and reduces the accumulation of salicylate (SA) in transgenic tobacco plants, the conversion of MeSA from SA leads to the depletion of the free SA pool. Overexpression of LcSAMT gene in tobacco significantly increases sensitivity of transgenic plants to drought stress, probably due to the decreased SA accumulation. Increased accumulation of ROS, elevated MDA levels, reduced proline contents, and lowered expression of APX, CAT and SOD genes are also observed in the LcSAMT transgenic tobacco plants under drought stress, which means that the LcSAMT-overexpressing transgenic tobacco plants have decreased resistance to oxidative stress in comparison with control plants under drought stress. LcSAMT-overexpressing transgenic tobacco plants display decreased abscisic acid (ABA) accumulation and reduced transcript expression of NtNCED1 and NtRD22 genes. Therefore, the increased sensitivity of transgenic plants overexpressing LcSAMT gene to drought stress might also act through an ABA-dependent pathway. Overexpression of LcSAMT decreases RWC, proline, chlorophyll content, and the photosynthetic capacity, and increases MDA content of transgenic Nicotiana tabacum plants exposed to drought stress |
756604 |
| 2.1.1.274 | malfunction |
recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leads to chlorosis and altered host susceptibility. Transcription of PbBSMT is associated with: (1) strong leaf phenotypes from anthocyanin accumulation and chlorosis followed by browning, (2) increased plant susceptibility after infection with Plasmodiophora brassicae that is manifested as more yellow leaves and reduced growth of upper plant parts, and (3) induced transgenic plants are not able to support large galls and had a brownish appearance of some clubs. Microarray data indicate that chlorophyll loss is accompanied by reduced transcription of genes involved in photosynthesis, while genes encoding glucose metabolism, mitochondrial functions and cell wall synthesis are upregulated. Phenotype overview |
757953 |
| 2.1.1.274 | metabolism |
expression patterns of Populus SABATH genes under normal growth conditions and abiotic stress, overview |
757984 |
