EC Number   |
General Information |
Reference |
|---|
   2.1.1.220 | evolution |
comparative structural analysis of TrmIs from archea, prokaryota, and eukaryota, overview |
719192 |
| 2.1.1.220 | evolution |
the m1A58 modification occurs on (cyt)tRNAs from all three domains of life and further in (mt)tRNAs. The enzyme belongs to the RFM class I methyltransferases. In eukaryotes, the m1A58 MTase located in the cytosol is composed of a catalytic protein unit from the Trm61 subfamily (Trm61A) and an RNA-binding protein unit from the Trm6 subfamily (Trm6). Trm6 and Trm61 share a common ancestor and arose via gene duplication and divergent evolution. The mitochondrial m1A58 MTase consists of a single protein from the Trm61 family (Trmt61B), which is a paralogue to Trm61A from the cytosolic complex |
756160 |
| 2.1.1.220 | evolution |
the m1A58 modification occurs on (cyt)tRNAs from all three domains of life and further in (mt)tRNAs. The enzyme belongs to the RFM class I methyltransferases. In eukaryotes, the m1A58 MTase located in the cytosol is composed of a catalytic protein unit from the Trm61 subfamily (Trm61A) and an RNA-binding protein unit from the Trm6 subfamily (Trm6). Trm6 and Trm61 share a common ancestor and arose via gene duplication and divergent evolution. The mitochondrial m1A58 MTase consists of a single protein from the Trm61 family (Trmt61B), which is a paralogue to Trm61A from the cytosolic complex. In mitochondria, MTase Trmt61B forms a tetramer, presumed to resemble the homotetramers of TrmI proteins. In support of a similar structural arrangement between Trmt61B and TrmI, a phylogenetic analysis confirmed a bacterial origin of the human protein |
756160 |
| 2.1.1.220 | evolution |
the m1A58 modification occurs on (cyt)tRNAs from all three domains of life and further in (mt)tRNAs. The m1A58 MTases belong to the RFM methyltransferase superfamily, class I. In archaea and bacteria, the m1A58 MTases belong to the TrmI subfamily and function without complex partners |
756160 |
| 2.1.1.220 | evolution |
the N1-methyladenosine residue at position 58 of tRNA is found in the three domains of life |
736707 |
| 2.1.1.220 | evolution |
TRM61 is the eukaryotic homologue of the bacterial and archaeal m1A58 tRNA methyl-transferase TrmI. Evolutionary relationship between TRM6 and TRM61, overview |
-, 758373 |
| 2.1.1.220 | malfunction |
deletion of the MTase N1-methylation A58 in yeast produces non-viable cells |
756160 |
| 2.1.1.220 | malfunction |
inactivation of the Thermus thermophilus trmI gene results in a thermosensitive phenotype (growth defect at 80°C) |
-, 660026 |
| 2.1.1.220 | malfunction |
siRNA knockdown of either subunit of the m1A58-methyltransferase results in a slow-growth phenotype, and a marked increase in the amount of m1A58 hypomodified tRNAs. Most m1A58 hypomodified tRNAs can associate with polysomes in varying extents |
721024 |
| 2.1.1.220 | malfunction |
the absence of m1A from all tRNAs in Saccharomyces cerevisiae mutants lacking Gcd10p elicits severe defects in processing and stability of initiator methionine tRNA |
485272 |
