    1.7.7.2 | malfunction |
the narB knockout of Synechococcus sp. 7002 is sufficient to redirect the dominant flux of electrons from nitrate reduction to other fermentative metabolites, including a 6fold higher H2 yield than in the wild-type strain. The DELTAnarB mutant is able to take up intracellular nitrate but is unable to reduce it to nitrite or ammonia, and is unable to grow photoautotrophically on nitrate. During photoautotrophic growth on urea, mutant DELTAnarB significantly redirects biomass accumulation into glycogen at the expense of protein accumulation. Because there is no metabolism of nitrate in the DELTAnarB strain, nitrate cannot act as a strong terminal electron acceptor to lower the cellular redox poise, which is known to inhibit glucose-6-phosphate dehydrogenase (G6PDH) activity. Phenotype, overview |
742142 |
