EC Number   |
General Information |
Reference |
|---|
  1.14.99.52 | evolution |
cyanobacterial OvoA homologues (Egt-B(ovo)) have evolved to catalyze an EgtB-type reaction by convergent evolution, cf. EC 1.14.99.50, in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide |
-, 744709 |
| 1.14.99.52 | evolution |
cyanobacterial OvoA homologues (Egt-B(ovo)) have evolved to catalyze an EgtB-type reaction by convergent evolution, cf. EC 1.14.99.50, in a competitive reaction containing 1 mM of each histidine, N-alpha-trimethylhistidine, and cysteine, OvoAErw-NW and EgtB(ovo) produce exclusively gamma-L-glutamyl-S-(hercyn-2-yl)-L-cysteine S-oxide. Because Erwinia tasmaniensis and most other OvoA encoding organisms do not encode an EgtD-type histidine methyltransferase, it seems clear that this N-alpha-trimethylhistidine-consuming side activity of OvoAErwin has no physiological purpose. Such promiscuity may have facilitated the transition of an ancestral sulfoxide synthase from ovothiol to erothioneine biosynthesis |
744709 |
| 1.14.99.52 | evolution |
enzyme OvoA belongs to the 2-His-1-carboxylate catalytic triad type of mononuclear non-heme iron enzymes |
-, 734828 |
| 1.14.99.52 | evolution |
OvoA and EgtB, EC 1.14.99.50, are related in sequence, while they are biochemically distinct |
743388 |
| 1.14.99.52 | evolution |
OvoA evolved in most marine metazoans and was lost in bony vertebrates during the transition from the aquatic to terrestrial environment. Evolutionary history of OvoA in metazoans, overview |
746446 |
| 1.14.99.52 | evolution |
OvoA evolved in most marine metazoans and was lost in bony vertebrates during the transition from the aquatic to terrestrial environment. The DinB superfamily domain contains the conserved HX3HXE putative iron-binding motif. The residues 581-587, 602-603, 661-663, and 680, considered to be involved in the formation of SAM-binding site, are conserved with the bacterial orthologous gene. OvoA shares two protein domains with EgtB, the DinB superfamily domain and the FGE-sulfatase domain but differs for the additional C-terminal putative SAM-transferase domain. Evolutionary history of OvoA in metazoans, overview |
746445 |
| 1.14.99.52 | evolution |
the enzyme belongs to the sulfoxide synthases |
-, 733647 |
| 1.14.99.52 | evolution |
the two known sulfoxide synthases EgtB and OvoA distinguish themselves from each other by their substrate preferences and product C-S bond regioselectivity |
-, 734827 |
| 1.14.99.52 | malfunction |
mutation of any of the residues in the HX3HXE motif in OvoA results in an over 100fold attenuation of activity |
745390 |
| 1.14.99.52 | malfunction |
mutation of the 2-His-1-carboxylate catalytic triad of the enzyme disrupts the cysteine dioxygenase activity |
-, 734828 |
