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EC Number General Information Commentary Reference
Display the reaction diagram Show all sequences 1.14.19.56physiological function enzyme forms part of the pyoluteorin biosynthesis gene cluster which includes type I polyketide synthases (pltB, pltC), an acyl coenzyme A (acyl-CoA) dehydrogenase (pltE), an acyl-CoA synthetase (pltF), a thioesterase (pltG), and three halogenases (pltA, pltD, and pltM). PltA gene disruption abolishes pyoluteorin production -, 745200
Display the reaction diagram Show all sequences 1.14.19.56physiological function the antifungal natural product pyoluteorin contains a 4,5-dichloropyrrole moiety. Dichlorination in the heteroaromatic ring occurs after proline is tethered by thioester linkage to the carrier protein PltL and enzymatically desaturated to the pyrrolyl-S-PltL. The FADH2-dependent halogenase PltA catalyzes chlorination at both positions of the ring, generating the 5-chloropyrrolyl-S-PltL intermediate and then the 4,5-dichloropyrrolyl-S-PltL product. PltA activity strictly depends on a heterologous flavin reductase that uses NAD(P)H to produce FADH2 -, 746280
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