EC Number   |
General Information |
Reference |
|---|
  1.14.14.38 | physiological function |
bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava |
708971 |
| 1.14.14.38 | physiological function |
bifunctional enzyme, metabolizes L-valine as well as L-isoleucine, i.e. activities of EC 1.14.14.38 and 1.14.14.39, consistent with the cooccurrence of linamarin and lotaustralin in cassava. CYP79D1 has a higher kcat value with L-valine as substrate than with L-isoleucine, which is consistent with linamarin being the major cyanogenic glucoside in cassava |
708971 |
| 1.14.14.38 | physiological function |
CYP79D1 and CYP79D2 are the two paralogous genes encoding the first committed enzymes in linamarin and lotaustralin synthesis. Blocking expression by RNA interference results in lines with acyanogenic leaves. Only a few of these lines are depleted with respect to cyanogenic glucoside content in tubers. Cyanogenic glucosides are synthesized in the shoot apex and transported to the root, resulting in a negative concentration gradient basipetal in the plant with the concentration of cyanogenic glucosides being highest in the shoot apex and the petiole of the first unfolded leaf |
-, 710305 |
| 1.14.14.38 | physiological function |
enzyme catalyzes the conversion of Val and Ile to the corresponding aldoximes in biosynthesis of cyanogenic glucosides and nitrile glucosides in Lotus japonicus. Recombinantly expressed isoforms CYP79D3 and CYP79D4 in yeast cells show higher catalytic efficiency with L-Ile as substrate than with L-Val, in agreement with lotaustralin and rhodiocyanoside A and D being the major cyanogenic and nitrile glucosides in Lotus japonicus |
710304 |
| 1.14.14.38 | physiological function |
in Lotus japonicus expressing Manihot esculenta CYP79D2, the the cyanide potential is approximately twice as high as in wild-type plants. While thelinamarin content is increased approximately 20fold, the lotaustralin content is only slightly increased. The ratio of rhodiocyanoside A and D to lotaustralin is unaltered in leaves. In roots expressing cassava CYP79D2, linamarin and lotaustralin can be detected although in much smaller quantities than in green tissue |
710304 |
| 1.14.14.38 | physiological function |
transgenic plants in which the expression of CYP79D1/CYP79D2 genes is selectively inhibited in leaves by antisense expression of CYP79D1/D2 gene fragments have 60-94% reduced linamarin leaf levels. These plants also have a greater than a 99% reduction in root linamarin content. Transgenic plants in which the CYP79D1/D2 transcripts are reduced to non-detectable levels in roots have normal root linamarin levels. Linamarin synthesized in leaves is transported to the roots and accounts for nearly all of the root linamarin content. Transgenic plants having reduced leaf and root linamarin content are unable to grow in the absence of NH3 |
710288 |
| 1.14.14.38 | physiological function |
transgenic plants in which the expression of CYP79D1/D2 genes is selectively inhibited in leaves by antisense expression of CYP79D1/D2 gene fragments have 60-94% reduced linamarin leaf levels. These plants also have a greater than a 99% reduction in root linamarin content. Transgenic plants in which the CYP79D1/D2 transcripts are reduced to non-detectable levels in roots have normal root linamarin levels. Linamarin synthesized in leaves is transported to the roots and accounts for nearly all of the root linamarin content. Transgenic plants having reduced leaf and root linamarin content are unable to grow in the absence of NH3 |
710288 |
