EC Number   |
General Information |
Reference |
|---|
    1.1.1.9 | evolution |
the conserved coenzyme binding motif (GxGxxG) and zinc-ADH signature (GHExxGxxxxxGxxV) are observed in the amino acid sequence of RpXDH at position 181-186 and 70-84 and are completely conserved among RpXDH, XDHs, and SDHs from other filamentous fungi and yeasts |
-, 742112 |
| 1.1.1.9 | evolution |
the enzyme belongs to a medium-chain dehydrogenase/reductase (MDR) superfamily and a subfamily of polyol dehydrogenase, PDH |
-, 738728 |
| 1.1.1.9 | evolution |
the enzyme belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily and polyol dehydrogenase (PDH) subfamily. The enzyme contains the typical NAD+-binding motif GxGxxG of MDR family enzymes |
-, 761551 |
| 1.1.1.9 | evolution |
the enzyme contains a NAD(P)-binding motif and a classical active site motif belonging to the short-chain dehydrogenase family |
-, 760272 |
| 1.1.1.9 | evolution |
XDH has a TGXXGXXG NAD(H)-binding motif and a YXXXK active site motif, and belongs to the short-chain dehydrogenase/ reductase family |
-, 742937 |
| 1.1.1.9 | malfunction |
deletin of gene xdhA and gene ladA and or both lead mutants with decreased dehydrogenase activities and increased xylitol production, overview |
-, 721913 |
| 1.1.1.9 | metabolism |
bioproduction pathway of xylitol, overview |
-, 722437 |
| 1.1.1.9 | metabolism |
biosynthesis of xylitol can be achieved from two distinctive routes, one occurs via the activity of NADPH-dependent xylose reductase (XR, EC 1.1.1.307), reducing xylose directly into xylitol. The other one proceeds via formation of the intermediate xylulose through xylose isomerase (XI, EC 5.3.1.5) followed by NADH-dependent reduction via the xylitol dehydrogenase (XDH). Both of the metabolic routes originate from xylose dissimilation and can lead to formation of xylulose-5-phosphtate, the entrance point of pentose phosphate pathway |
-, 760768 |
| 1.1.1.9 | metabolism |
in order to overcome xylitol accumulation in Aspergillus carbonarius, a K274R point mutation is introduced into the xylose reductase with the aim of changing the specificity toward NADH. Fermentation with the mutant strain (grown on D-xylose as the sole carbon source) shows a 2.8fold reduction in xylitol accumulation and 4.5fold increase in citric acid production compared to the wild-type strain. The fact that the mutant strain shows decreased xylitol levels is assumed to be associated with the capability of the mutated xylose reductase to use NADH generated by the xylitol dehydrogenase XDH, thus preventing the inhibition of XDH by the high levels of NADH and ensuring the flux of D-xylose through the pathway |
-, 743029 |
| 1.1.1.9 | metabolism |
key enzymes for xylitol production in yeasts are xylose reductase and xylitol dehydrogenase, overview |
699368 |
