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EC Number
General Information
Commentary
Reference
evolution
in general, DNA recombination in Escherichia coli accompanies the conversion of double-stranded DNA to single-stranded DNA by exonuclease. It is reported that Escherichia coli has at least seven exonucleases that prefer double-stranded DNA for their substrates: XthA, RecE, ExoX, RecBCD, SbcCD, Nfo, and TatD. In addition, YgdG is an exonuclease with yet undetermined substrate preference. It seems likely that Escherichia coli K-12 originally acquired iVEC activity, and the iVEC activity was involved in an unknown physiological function in Escherichia coli
malfunction
deletion of the DNA polymerase domain of PolA does not completely abrogate iVEC activity
metabolism
involvement of DNA polymerases in in vivo cloning of Escherichia coli (iVEC) activity, analysis of iVEC activities of various strains, which are deletion mutants of nonessential polymerases in the Keio collection, overview. XthA plays a critical role in the iVEC activity
more
enzyme residues E57 and D251 are critical for catalysis, molecular modelling and mutational analysis. Determinants of abasic-site recognition, overview. Homology modeling of MtbXthA using the structure of the Neisseria meningitidis protein (PDB ID 2JC4) as a template
more
enzyme residues E57 and D251 are critical for catalysis, molecular modelling and mutational analysis. Determinants of abasic-site recognition, overview. Homology modeling of MtbXthA using the structure of the Neisseria meningitidis protein (PDB ID 2JC4) as a template; the PIP motif mediates critical interactions between AP endonuclease and proliferating cell nuclear antigen (PCNA), both in vitro and in vivo. The PIP motif in PCNA-interacting proteins is a defined consensus sequence (QxxLxxFF), while the consensus sequence corresponding to the beta-clamp interacting motif in prokaryotes is relatively less conserved. Structure comparison of homodimeric mycobacterial beta-clamp and homotrimeric human PCNA, overview
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more
the PIP motif mediates critical interactions between AP endonuclease and proliferating cell nuclear antigen (PCNA), both in vitro and in vivo. The PIP motif in PCNA-interacting proteins is a defined consensus sequence (QxxLxxFF), while the consensus sequence corresponding to the beta-clamp interacting motif in prokaryotes is relatively less conserved. Structure comparison of homodimeric mycobacterial beta-clamp and homotrimeric human PCNA, overview
physiological function
Escherichia coli has an ability to assemble DNA fragments with homologous overlapping sequences of 15 to 40 bp at each end. In vivo cloning of Escherichia coli (iVEC) is independent of both RecA and RecET recombinases but is dependent on XthA, a 3' to 5' exonuclease. XthA resects the 3' ends of linear DNA fragments that are introduced into Escherichia coli cells, resulting in exposure of the single-stranded 5' overhangs. Then, the complementary single-stranded DNA ends hybridize each other, and gaps are filled by DNA polymerase I, molecular iVEC mechanism, overview. XthA helps to repair minor DNA damage, instead of the RecBCD exonuclease. RecBCD produces a 3' overhang and loads RecA onto the single-stranded DNA, causing an SOS response accompanied by cell division arrest. To help avoid such a serious outcome, it is conceivable that XthA functions in a repair pathway of DNA damage
physiological function
exonuclease III (Exo III) is a sequence-independent 3'-5' exonuclease
physiological function
exonuclease III (Exo III) plays crucial roles in maintaining the genome stability
physiological function
key enzyme involved in the repair of abasic sites and DNA strand breaks
Results 1 - 10 of 14 > >>