EC Number   |
General Information |
Reference |
|---|
   2.3.1.75 | evolution |
among six possible predicted Euglena WSD orthologues, gene expression analysis and in vivo evaluation for enzyme activity with yeast expressing individual recombinant WSDs indicates that two of them, EgWSD2 and EgWSD5, predominantly function as wax ester synthase. The six WSD isoforms in Euglena gracilis all contain WES and DUF1298 motifs |
758397 |
| 2.3.1.75 | evolution |
distinct evolutionary origin of Thraustochytrium roseum bifunctional wax ester synthase/acyl-CoA diacylglycerol acyltransferases (WS/DGATs) from that of plant and bacterial WS/DGATs. Both TrWSD4 and TrWSD5 contain the conserved acyltransferase active-site motif (HHXXXDG), whose first histidine residue is substituted to serine and aspartate, respectively |
-, 756245 |
| 2.3.1.75 | evolution |
distribution of putative WS/DGAT in Streptomyces genomes, overview |
755845 |
| 2.3.1.75 | malfunction |
a 5567-bp insertion of the enzyme gene in glossy Chinese cabbage variant SD369 causes a loss of protein function, thus blocking the very long chain fatty acid elongation of C26 to C28, and ultimately resulting in the glossy phenotype |
777746 |
| 2.3.1.75 | malfunction |
remarkably reduced wax ester contents in EgWSD2/5-double knockdown Euglena gracilis cells treated with anaerobic conditions. The decreased ability to produce wax ester does not affect adaptation of Euglena gracilis to anaerobiosis. Lipid profile analysis suggests an allocation of metabolites to other compounds including triacylglycerol instead of wax esters, lipid profile analyses of EgWSD2/5 double-knockout cells, effect of EgWSD gene silencing on wax ester production, overview. The relative amount of wax esters, including C28, is significantly low in EgWSD2/5 double-KD cells, whereas that of fatty acids does not differ between the silenced cells and mock control cells |
758397 |
| 2.3.1.75 | malfunction |
the global absence of AWAT2 enzymatic activity does not affect gross retinal morphology or the rate of visual chromophore regeneration by the canonical retinal pigment epithelium-specific 65 kDa protein-dependent visual cycle. There are reduced maximal rod and cone dark-adapted responses in enzyme-deficient mice compared to control mice. While rod dark adaptation is not affected by the lack of enzyme, M-cone dark adaptation both in isolated retina and in vivo is significantly suppressed |
777300 |
| 2.3.1.75 | malfunction |
the respective gene, atfG25, is inactivated in Streptomyces sp. strain G25, cells of the insertion mutant still exhibit DGAT activity and are able to store TAG, albeit in lower quantities and at lower rates than the wild-type strain |
755845 |
| 2.3.1.75 | malfunction |
the wfl gene mutants (wrinkled flower and leaf) show sparse epicuticular wax crystals on the leaf surface and increased water permeability in leaves compared to the wild type. The mutation of the enzyme leads to a markedly increased percentage of C20:0, C22:0, and C24:0 fatty acids in leaves, a significantly reduced cuticular wax content, and dramatic changes of wax constituents in leaves |
778979 |
| 2.3.1.75 | metabolism |
the enzyme plays a pivotal role in wax ester synthesis |
758397 |
| 2.3.1.75 | more |
quantitative expression analysis of WS and WSD genes in response to aerobic and anaerobic conditions |
758397 |
