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physiological function
the tylH mutant, which is blocked in the oxidation of C-23 methyl to hydroxymethyl, is capable of carrying out all other biosynthetic steps except addition of 6-deoxy-D-allose and subsequent O-methylations and produces the biologically active compound 23-deoxydemycinosyl tylosine. A tyID tylH lacking double mutant, blocked specifically in the biosynthesis of 6-deoxy-D-allose and in specific oxidation of tylactone, accumulates 23-deoxydemycinosyl tylosin and therefore cannot oxidize the C-23 methyl position of lactone
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