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Expression
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cloning of the fused Mycobacterium smegmatis fbiC gene and heterologous expression of the bifunctional MsFbiC in Escherichia coli
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heterologous expression in Escherichia coli and functional characterization in vitro. MjCofH is expressed as a fusion protein with an N-terminal polyhistidine tag. The His-tagged protein is strongly expressed in Escherichia coli but is slightly more soluble than the untagged protein
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