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cloning of the fused Mycobacterium smegmatis fbiC gene and heterologous expression of the bifunctional MsFbiC in Escherichia coli
heterologous expression in Escherichia coli and functional characterization in vitro. MjCofH is expressed as a fusion protein with an N-terminal polyhistidine tag. The His-tagged protein is strongly expressed in Escherichia coli but is slightly more soluble than the untagged protein
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