6.3.2.4 | ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine |
first, the ATP-dependent dimerization reactions catalyzed by DdlA proceed through the phosphorylation of the D-alanine at the carboxyl group by the gamma-phosphate of ATP to produce an acylphosphate intermediate. Then, a tetrahedral intermediate is formed by the nucleophilic attacking of the amino nitrogen of the second D-alanine on the acylphosphate intermediate. Finally, a phosphate group is released from the tetrahedral intermediate to generate the final product of D-alanyl-D-alanine |
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