EC Number |
Reaction |
Reference |
---|
6.2.1.13 | ATP + acetate + CoA = ADP + phosphate + acetyl-CoA |
- |
- |
6.2.1.13 | ATP + acetate + CoA = ADP + phosphate + acetyl-CoA |
reaction mechanism and structural modelling, formation of enzyme-bound acetyl phosphate and enzyme phosphorylation at His257alpha, overview |
693061 |
6.2.1.13 | ATP + acetate + CoA = ADP + phosphate + acetyl-CoA |
reaction mechanism: the formation of enzyme-bound acetyl phosphate and enzyme phosphorylation at His-257alpha, respectively, proceeds in analogy to succinyl-CoA synthetases. In contrast to succinyl-CoA synthetases, in acetyl-CoA synthetase the phosphoryl group is transferred from the His-257alpha to ADP via transient phosphorylation of a second conserved histidine residue in the beta-subunit, His-71alpha. It is proposed that acetyl-CoA synthetase reaction follows a four-step mechanism including transient phosphorylation of two active site histidine residues |
693061 |
6.2.1.13 | ATP + acetate + CoA = ADP + phosphate + acetyl-CoA |
the enzyme uses a four-step reaction mechanism including an additional phosphoryl transfer step, the important His252, located in the beta subunit, is invovled |
744911 |