EC Number |
Reaction |
Reference |
---|
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
- |
- |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
A293 is important for the stability of the enzyme conformation and the editing function and probably is involved in the ATP binding |
650024 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
active site structure and mechanism, the editing active site binds the two different substrates using a single amino acid discriminatory pocket while preserving he same mode of adenine recognition, Asp347 is involved in the editing process, mechanism of hydrolysis |
653192 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
Asp345 is involved in the editing process, mechanism of hydrolysis |
653192 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
Asp419 is involved in the editing process, mechanism of hydrolysis |
653192 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
ATP and tRNA are bound to the enzyme in almost random order, and diphosphate is dissociated before the rate-limiting step |
417 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
bi uni uni bi ping-pong mechanism with ordered addition of ATP and leucine and random release of AMP and leucyl-tRNALeu |
428 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
each amino acid is bound through its carboxyl group to the terminal nucleotide (2'- or 3'-hydroxyl end) of specific polynucleotide chains |
422 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
recognition of the tRNALeu requires the discriminator base A73 and the long variable arm of appropriate stem length, especially the Haloferax volcanii-specific loop sequence A47CG47D and U47H at the base of the helix |
652803 |
6.1.1.4 | ATP + L-leucine + tRNALeu = AMP + diphosphate + L-leucyl-tRNALeu |
residue E292 is important for aminoacylation activity |
653045 |