5.1.99.4 | (2S)-2-methylacyl-CoA = (2R)-2-methylacyl-CoA |
reaction mechanism, overview, the catalysis of the 1,1-proton transfer by alpha-methyl-acyl-CoA racemase is coupled to a movement of the fatty acyl moiety over a hydrophobic, methionine-rich surface, the mechanism involves the acid/base-pair residues His126 and Asp156, active site geometry, the thioester oxygen atom and the 2-methyl group are in a cisconformation with respect to each other, the thioester oxygen atom fits into an oxyanion hole and the 2-methyl group points into a hydrophobic pocket, Asp127A is a prominent residue in the active site and is located at the bottom of the catalytic site |
681420 |