EC Number   |
Reaction |
Reference |
|---|
    4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
- |
- |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
catalytic and kinetic mechanism, catalyzes carboligation as side reaction |
650144 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
catalytic mechanism |
-, 649662, 650009, 650049, 650541, 651124 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
catalytic mechanism, catalyzes the carboligation of 2 aldehydes as side reaction, mechanism |
650079 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
catalyzes the carboligation of 2 aldehydes as a side reaction, mechanisms of both reactions |
650671 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
catalyzes the carboligation of 2 aldehydes as a side reaction, mechanisms of both reactions, thermodynamic data |
650671 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
enzyme forms covalent intermediate C2-alpha-lactylthiamine diphosphate. Rate of reaction is limited by release of thiamine diphosphate |
665596 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
Glu51 is the most important residue in formation of the ylide and the release of acetaldehyde. Glu477 and Asp28 are involved in decarboxylation of lactylthiamine diphosphate. Protonation of alpha-carbanion to form 2-(1-hydroxyethyl)-thiamine diphosphate goes through a concerted double proton transfer transition state involving both Asp28 and His115. Decarboxylation of lactylthiamine diphosphate and protonation of alpha-carbanion are two rate-limiting steps |
666155 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
in absence of substrate, equilibrium is shifted to 4'-aminopyridine thiamine diphosphate. Carbonyl group of substrate forms a hydrogen bond to Tyr290, geometry of substrate is well-suited for a nucleophilic attack by ylide-thiamine diphosphate |
664077 |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 |
mechanism |
114286, 649851, 649910, 650152 |
